Figure 7.

³¹P solid-state NMR of LUVs incubated with Aβ_1-40. ³¹P chemical shift spectra of large unilamellar vesicles composed of 7:3 POPC/POPS (A) and 5.5:3:1.5 POPC/POPS/ganglioside (B) before (blue line) and after the addition of Aβ_1-40 (red line) are illustrated. The small peak near 0 ppm in the ganglioside-containing spectra indicates the formation of small, rapidly tumbling lipid structures indicative of membrane fragmentation. The absence of a corresponding peak for samples without ganglioside is an indication that membrane fragmentation does not occur. All spectra were obtained at 37°C and referenced with respect to 85% H₃PO₄ at 0.0 ppm.