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. 2012 Aug 15;186(4):359–368. doi: 10.1164/rccm.201201-0057OC

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Copyright © 2012 by the American Thoracic Society

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Figure 2. — Validation by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) of selected genes and miRNAs. Three signature genes (ADORA2B, C1QBP, ADC) and three miRNAs were selected for further validation by RT-qPCR. (A) Expression values of all three genes correlated with the changes in right ventricular systolic pressure (RVSP) and closely mirror the observed correlation by the microarray data. (B, C) Expression values of three miRNAs from the discovery cohort and the validation cohort, respectively, which correlated with the changes in RVSP and closely mirror the observed correlation by the microarray data.