Figure 3.

Gene expression validation. We used quantitative reverse transcriptase polymerase chain reaction (qPCR) to confirm expression patterns predicted by microarray analysis. We selected nine genes, based on their magnitude of change and biological interest. Shown are fold differences in expression (log scale) for each gene, comparing all samples, or samples stratified by gestational age. All genes demonstrated evidence for replication, with seven of nine displaying significant differences between bronchopulmonary dysplasia and controls. (*P value < 0.05; ^#P value < 0.10).