Figure 2. A PcG complex remains associated with templates through DNA replication with T7 bacteriophage proteins in the presence of double stranded DNA competitor.

A) Schematic of T7 replication experiments with PRC1. PRC1 is incubated with the template. Competitor DNA is added either with PRC1 (t1) or at the start of replication (t3). PRC1 bound and unbound templates are separated by sucrose gradient sedimentation. B–G)Phosphorimager scans of a T7 replication experiment. Reactions B–E were analyzed under denaturing conditions so bands show full length replication products; F and G (which are not replicated) were electrophoresed under native conditions since the labeled primer is not extended in the absence of T7 proteins.