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. 2012 Sep 17;2:661. doi: 10.1038/srep00661

Figure 3. PRC1 remains bound through T7 DNA replication in the presence of single stranded DNA competitor.

Figure 3

A) Schematic of T7 replication experiments with PRC1. PRC1 is incubated with the template. Competitor DNA is added either with PRC1 (t1) or at the start of replication (t3). PRC1 bound and unbound templates are separated by sucrose gradient sedimentation (repeated from Fig. 2). B–G) Phosphorimager scans of a representative T7 replication experiment carried out with single stranded DNA competitor. Reactions were carried out as in Figure 2. Reactions B–E were analyzed under denaturing conditions so bands show full length replication products; F and G (which are not replicated) were electrophoresed under native conditions since the labeled primer is not extended in the absence of T7 polymerase.