Table 2.
Sarcomere Shortening Parameters
| ||||||
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Resting SL (um) | Shortening Amplitude (um) | Dep Velocity (%amp) | 50% Relaxation Time (s) | 75% Relaxation Time (s) | 90% Relaxation Time (s) | |
NTG | 1.835±0.008 | 0.103±0.009 | 19.69±0.49 | 0.0689±0.002 | 0.107±0.004 | 0.18±0.01 |
R193H | 1.675±0.01* | 0.091±0.004 | 20.85±0.49 | 0.104±0.004* | 0.209±0.008* | 0.39±0.015* |
Ca2+ Transient Parameters
| ||||||
---|---|---|---|---|---|---|
Diastolic Ca2+ (360/380) | Ca2+ Amplitude (360/380) | Rise Velocity (%amp) | 50% Decay Time (s) | 75% Decay Time (s) | 90% Decay Time (s) | |
NTG | 1.14+0.02 | 0.115+0.012 | 87.7+8.6 | 0.141+0.004 | 0.266+0.012 | 0.42+0.03 |
R193H | 0.09+0.02 | 0.055+0.004* | 105.2+10.5 | 0.139+0.005 | 0.250+0.011 | 0.40+0.02 |
Departure and Ca2+ transient rise velocity is normalized to shortening and Ca2+ amplitude. Relaxation and Ca2+ decay time is calculated from peak shortening to 50, 75, or 90% of relaxation.
Values are represented as the mean ± SEM,
P<0.05 significantly different, t-test, n = 30 myocytes from 5 preparations.
Key: SL=Sarcomere Length, Dep Velocity = sarcomere length shortening velocity, and Rise Velocity = rate of rise of the Ca2+ transient.