Figure 4.

Tissue-localization of maize AChE in the coleoptile node and the mesocotyl. Immunofluorescence images of cross sections (6 μm) of the paraffin-embedded coleoptile node (A–F) and mesocotyl (G–L). (A, C, G and I) secondary antibody alone (negative control). (B, D–F, H and J–L) anti-maize AChE staining. (E, F, K and L) magnified images of the regions highlighted by dashed line boxes in (B, D, H and J) respectively. Arrowheads indicate localization of maize AChE. En, endodermis; Ph, phloem; Xy, xylem. Bars = 100 μm for (A–D and G–J); 10 μm for (E, F, K and L).