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. 2012 Mar 1;7(3):349–360. doi: 10.4161/psb.18967

Figure 2.

Figure 2.

NO content in maize roots and dry weights of shoots or roots in response to exogenously applied NO and NaCl treatment. NO content (A and B) were measured on maize plants treated at the V1 stage for a period of 21 d, with treatments done every three days. In panel A, fluorescence intensity was measured, using the AlphaEase FC imaging software, following pre-incubation with 400 μM cPTIO for 30 min (B2), treatment with 10 μM DETA (B3), 10 μM DETA/NO (B4), 150 mM NaCl (B5), 10 μM DETA + 150 mM NaCl (B6), 10 μM DETA/NO + 150 mM NaCl (B7), with autofluorescence intensity also measured from roots obtained from untreated plants without the DAF-2 DA fluorescence probe (B1). In panel B, No content was measured using a hemoglobin-based spectrophotometric assay. Biomass was also evaluated by measuring shoot (C) and root (D) dry weights at the end of the treatment period. Bars represent the means ± standard errors (SE) of three independent experiments with two plants used for each experiment to measure NO content and six plants per experiment for determining dry weights.