Figure 2.

Images show the average fluorescence intensity of L5 petiole cross sections following L1 petiole (A) or root (B) application at 30 and 90 min. Plants were treated with CF dye, Alexa Fluor 488 BSA and Alexa Fluor 488 Histone H1. Bar = 500 μm. Arrows point to fan-shaped bundles and arrowheads point to small circular bundles. (C) and (D) graphically depict the average fluorescence values per cross section (n = 4). Fluorescence values of three fan-shaped bundles and two circular bundles per petiole cross section were measured using ImageJ software and were added as a sum measure of fluorescence. All values reach a maximum at 90 min. Uptake of Alexa-BSA and Alexa-Histone are higher when delivered to the roots than to the L1 petiole. All protein values were lower than CF dye.