Figure 3. Inhibition of ATM and PARP is more cytotoxic in cells in which p53 function has been disrupted.

1. Z138 (grey bars), JVM-2 (black bars), HBL-2 (white bars) and UPN1 (hatched bars) were treated with DMSO (Untreated), KU55933 (5 µM), or olaparib (5 µM) or both KU55933 and olaparib (KU/Olaparib; both at 5 µM) for 96 h. Results were analysed by Student's t-test. n = 3, p-values were 0.013 for UPN1 versus Z138, 0.037 for UPN1 versus JVM-2, 0.0007 for Z138 versus HBL-2 and 0.0015 for Z138 versus JVM2. Results were considered statistically significant when p < 0.05, marked by *.
2. Human BJ fibroblast cells and BJ cells expressing HPV genes E6/E7 were exposed to DMSO (grey bars), KU55933 (5 µM; black bars), olaparib (5 µM; while bars) or both KU55933 and olaparib (KU/Olaparib; both at 5 µM) for 96 h. In both panels, cell viability was determined by trypan blue exclusion and normalized to DMSO treated controls for each cell line. Results were analysed by Student's t-test, n = 3. p-Values were 0.00049 for KU55993/Olaparib versus DMSO, 0.00049 for KU55933/Olaparib versus KU55933 and 0.00174 for KU55933/Olaparib versus Olaparib. Results were considered statistically significant when p < 0.05, marked by an *.