TABLE 1.
Primers used in this study
| Primer | Sequencea |
|---|---|
| VC2338_A | CATCACCCTAAGCAGCAG |
| VC2338_B | TTTACTCCTCGGCTTGCCACAATAAGCCAGAG AGC |
| VC2338_C | ATTGTGGCAAGCCGAGGAGTAAAGAAG |
| VC2338_D | GCCAGACCACCACGATGATAACCAATC |
| VCA0952_A | GTCCCATGGCGATATTGTGGGTGGACGC |
| VCA0952_B | TGTTAGGAGTCTCTTCGAGTTTTAGTTCAATA GGC |
| VCA0952_C | CTCGAAGAGACTCCTAACACATCAAGGCTAAC ATG |
| VCA0952_D | CTCGAGCTCGATACGCTATGAATAAATTGAGT GGTAAG |
| VCA0952_rev_prom | GCGCTATCTTTTGTTTACTTGACGC |
| VC0665_AII | CCGCCATGGGCTCCTGACCAATACTCACACTA TCCG |
| VC0665_BII | GTTGCACGCGCAGTCTCGACAAATTACGCATC GG |
| VC0665_C | TTCCGTATGGCGCGTGCAACCATGTATCGC |
| VC0665_D | CTGGAGCTCGCTCAAATCATTGCCCATTGCG |
| vpsL_prom_5′ | GGATCCTGAGTGAGTGACATATTGTTCTGTTT TTCCT |
| vpsL_prom_3′ | GGATCCTTAGTTAGTTAGTACTGAATCCATA CGGAAT |
| vpsA_prom_5′ | GGATCCTGAGTGAGTGATTTCTTAGCAAGGCG AAT |
| vpsA_prom_3′ | GGATCCTTAGTTAGTTACAGAGGTGCCATTTT GAT |
| vpsR_prom_5′ | GGATCCTGAGTGAGTGAACGATGCTGAAGAC CAAGAT |
| vpsR_prom_3′ | GGATCCTTAGTTAGTTAGTACTGAATCCATAC GGAAT |
| lacZrev4 | GCCAGTGAATCCGTAATCATGGTC |
a
Restriction sites are underlined and additional primer sequences that anneal with another primer sequence are shown in italics.