FIG. 2.

Northern blot analysis of the idnK, idnD, idnO, idnT, and IdnR transcripts in E. coli W1485. Total RNA was isolated from late-log-phase cultures grown on MOPS minimal medium containing the carbohydrate listed above each lane. A total of 5 μg of RNA was loaded per lane. Estimated transcript sizes (in kilobases) are shown to the right of each blot and were determined from an RNA Millennium Marker (Ambion, Inc.) run with each independent RNA gel (data not shown). Hybridizations were carried out with 300-nucleotide probes specific for the gene encoding the protein indicated under each blot.