TABLE 1.
E. coli strains used in this study
| Strain | Relevant genotype, phenotype, or characteristics | Reference or source |
|---|---|---|
| W1485 | K-12 wild type (λ−rph-1) | CGSCa |
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 Δ(araBAD)AH33 Δ(rhaBAD)LD78 | 6 |
| BW25141 | lacIqrrnBT14 ΔlacZWJ16hsdR514 Δ(araBAD)AH33 Δ(rhaBAD)LD78galU95 endABT333 uidA [(ΔmluI)::pir+recA1] | 6 |
| CB130 | λ−rph-1 Δ(lacI′b-PlacZ)c PidnidnK-lacZd; Kmr | This study |
| CB131 | λ−rph-1 Δ(lacI′-PlacZ)c PidnidnD-lacZd; Kmr | This study |
| CB360 | λ−rph-1 idnD::kan | This study |
| CB361 | λ−rph-1 ΔidnD | This study |
| CB361Z | λ−rph-1 ΔidnD Δ(lacI′-PlacZ)cidnD-lacZd | This study |
| CB366 | λ−rph-1 ΔidnR | This study |
| CB370 | λ−rph-1 Δcrp | This study |
| CB371 | λ−rph-1 ΔgntR | This study |
| DH5α | supE44 ΔlacU169(lacZΔM15Δ)hsdR17 endA1 gyrA96 thi-1 relA1 | BRLe |
| MD5 | λ−rph-1 idnK::kan | Fernando Valle |
| MD5E | λ−rph-1 idnK::kan gntK::cat | Fernando Valle |
| NP202 | λ−rph-1 Δ(gntRKU) | This study |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
a
CGSC, E. coli Genetic Stock Collection.
b
Truncated gene (′).
c
Δ(lacI′-PlacZ) removes the 3′ terminus of lacI, the entire intergenic regulatory region upstream of lacZ, and the 5′-terminal region of lacZ.
d
The lacZ coding region is fused in frame to the 5′ region of the indicated idnK or idnD gene, forming a translation fusion. (see Materials and Methods).
e
BRL, Bethesda Research Laboratories.