TABLE 2.
Plasmids used in this study
| Plasmid name | Description | Reference or source |
|---|---|---|
| pBluescriptIISK+ | Cloning vector; Apr | Stratagene |
| pSP72 | Cloning vector; Apr | Promega |
| pRS551 | Transcription fusion vector containing the MCS, EcoRI-SmaI-BamHI-lacZYA′a | 27 |
| pRS552 | Translation fusion vector containing the MCS, EcoRI-SmaI-BamHI-lacZYA′ | 27 |
| pRS577 | Translation fusion vector containing the MCS, BamHI-SmaI-EcoRI-lacZYA′ | 27 |
| pNP202 | Replaced a 1.3-kb StuI-BglII fragment on pTC221 with the Kanr gene cassette | This study |
| pCB92 | pBSKs+ containing 300-bp idnK′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB100 | pBSKs+ containing 300-bp idnD′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB200 | pBSKs+ containing 300-bp idnO′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB620 | pBSKs+ containing 300-bp idnT′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB700 | pBSKs+ containing 300-bp yigR′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB900 | pBSKs+ containing 300-bp idnR′ PstI-KpnI DNA fragment generated by PCR | This study |
| pCB108 | pSP72 containing 542-bp lacI′ PstI DNA fragment generated by PCR | This study |
| pCB551 | pCB108 containing 2,223-bp neo rrnB T14lacZ′ DNA fragment from pRS551 | This study |
| pCB552 | pCB108 containing 2,223-bp neo rrnB T14lacZ′ DNA fragment from pRS552 | This study |
| pCB577 | pCB108 containing 2,223-bp neo rrnB T14lacZ′ DNA fragment from pRS577 | This study |
| pCB120 | pCB552 containing EcoRI-BamHI fragment of 274-bp idn regulatory region | This study |
| pCB121 | pCB577 containing BamHI-EcoRI fragment of 274-bp idn regulatory region | This study |
| pCB220 | pCB551 containing EcoRI-BamHI fragment of 274-bp idn regulatory region | This study |
a
Truncated gene (′).