TABLE 4.
β-Galactosidase activity of CB130 (idnK-lacZ) and CB131 (idnD-lacZ) grown in MOPS complete medium
| Carbon sourceb | β-Galactosidase activity (Miller unitsa) with strain:
|
|
|---|---|---|
| CB130 | CB131 | |
| Idonate + cAMPc | 1,667 ± 169 | 1,816 ± 146 |
| Idonate | 750 ± 28 | 714 ± 33 |
| Idonate + gluconate | 441 ± 40 | 418 ± 33 |
| Gluconate | 279 ± 20 | 342 ± 19 |
| Gluconate + cAMPc | 405 ± 31 | 383 ± 43 |
| 5-Ketogluconate | 499 ± 62 | 495 ± 57 |
| 2,5-Diketogluconate | 157 ± 51 | 119 ± 22 |
| 2-Ketogluconate | 169 ± 25 | 108 ± 25 |
| Iduronate | 140 ± 10 | 155 ± 16 |
| 2-Ketogulonate | 25 ± 13 | 45 ± 11 |
| Glycerol | 104 ± 3.6 | 115 ± 18 |
| Glucose | 123 ± 8.2 | 116 ± 25 |
| Succinate | 155 ± 18 | 123 ± 34 |
a
Reported values are means and standard deviations of three independent experiments performed in triplicate.
b
Cells were grown in MOPS complete medium containing the carbon sources indicated at 0.2%.
c
Exogenous cAMP added to growth medium at 4mM.