TABLE 6.

The fifty most highly induced E. coli genes in cells grown on l-idonate

Gene	Gene product	Intensitya	Ratiob	P valuec
idnD	l-Idonate dehydrogenase	0.254	1.6	1.4 × 10−6
idnO	5-Ketogluconate:gluconate oxidoreductase	0.187	1.3	1.9 × 10−7
b2790	ORFd hypothetical protein	0.172	0.97	2.4 × 10−4
yheH	Putative export protein for general secretion pathway	0.041	0.93	9.1 × 10−4
idnK	Gluconate kinase	0.039	0.83	2.1 × 10−3
yfeK	ORF, hypothetical protein	0.055	0.81	4.8 × 10−4
yhfP	ORF, hypothetical protein	0.022	0.79	5.9 × 10−4
yicM	Putative transport protein	0.104	0.78	8.9 × 10−4
yhdT	ORF, hypothetical protein	0.035	0.78	5.0 × 10−5
narW	Cryptic nitrate reductase 2, delta subunit	0.019	0.73	5.4 × 10−4
flhA	Flagellar biosynthesis; possible export function	0.108	0.72	2.9 × 10−3
ymfL	ORF, hypothetical protein	0.021	0.72	1.6 × 10−4
rimJ	Acetylation of N-terminal alanine protein S5	0.124	0.69	2.6 × 10−8
ymfO	ORF, hypothetical protein	0.050	0.68	2.0 × 10−3
b2459	ORF, hypothetical protein	0.108	0.66	6.2 × 10−4
yggC	Putative kinase	0.031	0.60	3.8 × 10−4
narJ	Nitrate reductase 1, delta subunit, assembly function	0.068	0.60	8.9 × 10−5
narU	Nitrite extrusion protein 2	0.014	0.59	9.7 × 10−4
yjiW	ORF, hypothetical protein	0.017	0.58	5.0 × 10−4
yiaC	ORF, hypothetical protein	0.041	0.58	1.1 × 10−4
thiM	Hydoxyethylthiazole kinase	0.089	0.57	2.0 × 10−3
b1600	Possible chaperone	0.017	0.56	5.9 × 10−4
ycal	ORF, hypothetical protein	0.032	0.56	7.3 × 10−4
b1152	ORF, hypothetical protein	0.015	0.55	2.3 × 10−3
hyaF	Nickel incorporation into hydrogenase 1 proteins	0.045	0.54	2.5 × 10−5
b2460	ORF, hypothetical protein	0.061	0.54	2.5 × 10−3
ymfA	ORF, hypothetical protein	0.020	0.53	4.7 × 10−4
nrfE	Formate-dependent nitrite reductase, assembly function	0.381	0.53	2.3 × 10−3
yibK	ORF, hypothetical protein	0.027	0.53	2.8 × 10−3
aceE	Pyruvate dehydrogenase (decarboxylase component)	0.046	0.52	5.8 × 10−4
evgS	Putative sensor for regulator EvgA	0.205	0.51	7.4 × 10−7
ycjV	Putative ATP binding component of a transport system	0.088	0.51	6.9 × 10−5
queA	Synthesis of queuine in tRNA	0.080	0.51	2.7 × 10−3
idnR	l-Idonate operon regulator	0.045	0.51	1.2 × 10−3
idnT	l-Idonate transporter	0.064	0.51	6.0 × 10−5
yafV	Putative EC 3.5 amidase-type enzyme	0.023	0.51	1.7 × 10−3
yi21-2	IS2 hypothetical protein	0.057	0.50	1.3 × 10−3
cynT	Carbonic anhydrase	0.034	0.50	2.2 × 10−5
yhgG	ORF, hypothetical protein	0.019	0.50	1.5 × 10−4
b1565	ORF, hypothetical protein	0.011	0.50	6.6 × 10−10
yi21-6	IS2 hypothetical protein	0.046	0.49	1.3 × 10−3
yihN	Putative resistance protein (transport)	0.020	0.49	1.8 × 10−4
b2931	Putative oxidoreductase	0.097	0.48	2.7 × 10−3
ychM	ORF, hypothetical protein	0.160	0.48	3.2 × 10−5
b1141	ORF, hypothetical protein	0.080	0.48	2.3 × 10−3
trpD	Anthranilate synthase component II	0.177	0.48	1.2 × 10−5
yrdB	ORF, hypothetical protein	0.013	0.47	1.2 × 10−3
yhfY	ORF, hypothetical protein	0.019	0.47	4.0 × 10−5
entA	2,3-dihydroxybenzoate dehydrogenase, enterochelin	0.017	0.47	2.4 × 10−4
yjgH	ORF, hypothetical protein	0.085	0.46	1.4 × 10−4

^aIntensity normalized to transcript levels in cells grown on l-idonate and expressed as a percentage of the sum of the transcript levels of all of the genes in the arrays (5).

^bLog₁₀ ratio of normalized transcript levels on l-idonate compared to those on d-glucose.

^cEach P value (scientific notation) indicates the probability that the reported log₁₀ ratio is significant.

^dORF, open reading frame.