TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli JM109 | endA1 gyr96 thi hsdR71 supE44 recA1 relA1 Δ(lac-proAB) (F′ traD36 proAB+lacIqlacZΔM15) | 55 |
| A. tumefaciens A348 | Wild type, pTiA6NC | 23 |
| A. tumefaciens PC1001 | pTiA6NC carrying an in-frame deletion of virB1 | 8 |
| A. tumefaciens UIA143(pTiA6) | C58 derivative; recA ery140 pTiA6 Eryr | 9 |
| Plasmids | ||
| pTrc200 | Strr, Spcr, pVS1 origin, lacIq, trc promoter expression vector | 46 |
| pTrcB1 | pTrc200, carrying 759-bp NcoI/BglII virB1 fragment from A. tumefaciens C58 | 45 |
| pTrcB1E→A | pTrcB1, mutated A. tumefaciens virB1 gene, active-site Glu60 changed to Ala | This work |
| pTrcB1s | pTrc200, carrying 717-bp NcoI/ScaI virB1 fragment from B. suis | This work |
| pTrcB1sE→A | pTrcB1s, mutated B. suis virB1 gene, active-site Glu27 changed to Ala | This work |
| pTrcTraL | pTrc200, carrying 735-bp NcoI/ScaI traL fragment from pKM101 | This work |
| pTrcTraLE→A | pTrcTraL, mutated pKM101 traL gene, active-site Glu53 changed to Ala | This work |
| pTrc169 | pTrc200, carrying 507-bp NcoI/ScaI fragment from the F plasmid | This work |
| pTrc523 | pTrc200, carrying 507-bp NcoI/ScaI fragment from H. pylori chromosomal DNA | This work |
| pBAD18 | Carr, cloning vector | 24 |
| pT7-7 | Carr, cloning vector | 48 |
| pLS1 | Carr, IncQ plasmid for VirB/D4-mediated transfer experiments | 47 |
| pKM101 | Carr, mucA, mucB, IncN broad-host-range plasmid | 54 |
| pUCvirB | Carr, virB region from B. suis 1330 | 40 |