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Effects of deletion of hns and tolC on drug accumulation in E. coli cells. Strains W3104ΔacrAB (lanes 1), W3104ΔacrABΔhns (lanes 2), and W3104ΔacrABΔhnsΔtolC (lanes 3) were spotted onto L-agar plates containing 1 μg of ethidium bromide/ml (A and B) or 0.5 μg of rhodamine 6G/ml (C and D). After incubation at 37°C for 18 h, E. coli colonies were observed under visible white light (A and C) and UV light (B and D).
