TABLE 2.

Oligonucleotides used for construction and verification of deletion strains

Oligonucleotide	Oligonucleotide sequence (5′ to 3′)
acrA-No	CGCGGATCCATTCGCATTTGTGGAATATAATCTCCATCA
acrA-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATATGTAAACCTCGAGTGTCCG
acrB-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTGATACAACGTGTAATCACTAAGGCC
acrB-Co	CGCGGATCCATGGAAAAAACTTACTGACCTGGAC
emrK-No	CGCGGATCCTGGATACCGTTAACTCCGGGG
emrK-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCACTATTATCTCTCATTTCTCATAGAT
emrY-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTGATGATAAAAGGAGGGGGTTATAGCG
emrY-Co	CGCGGATCCTGTTGGCGGTGGTCCTGGTGG
mdtE-No	CGCGGATCCCAGTTCAAAATTATGCAACTGATTCTG
mdtE-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATTTTAGTCCCTGAAAATTCTTGAG
mdtF-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTAACGTGTAAATGAGAGTAAGGTTGA
mdtF-Co	CGCGGATCCCGTCAAATTCCTCTGCATACTATTGC
acrE-No	CGCGGATCCCGTCGTCTTGCTTACGCCAT
acrE-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATTACTATTCCTCAAAAAACCAAAAG
acrF-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTAAATCAGAAACATAAAGGCGCTTTCG
acrF-Co	CGCGGATCCCGTCGTCTTGCTTACGCCAT
tolC-No	CGCGGATCCTCATCCCGGCAACCATCTC
tolC-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATTCCTTGTGGTGAAGCAGTAT
tolC-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTGATGACGACGACGGGG
tolC-Co	CGCGGATCCGCTGGATTGCTGGGCC
hns-No	CGCGGATCCCTCCCTTACGAAGCCTTGCATAATCC
hns-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATTGTAGTAATCTCAAACTTATAT
hns-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTAATCTTTTGTAGATTGCACTTGC
hns-Co	CGCGGATCCGTTGAATTAGCGCCGGGTGAAAGCGTAC
evgA-No	CGCGGATCCGAAAACGCAATAAATAAAACTACCGCC
evgA-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATAGATTATTCCCTTTGCAATGA
evgS-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTAAATAGCGGCTCCCACAATGTTC
evgS-Co	CGCGGATCCCATGGCACCTTTTGATGTTTTCAATACT
ydeO-No	CGCGGATCCTATTCCGGTTGAATTAGAACC
ydeO-Ni	CACGCAATAACCTTCACACTCCAAATTTATAACCATTTTATCTCCTTAAAACAATAAAGT
ydeO-Ci	GTTATAAATTTGGAGTGTGAAGGTTATTGCGTGTGATTATTTGCTAACGAGTAGTCAACC
ydeO-Co	CGCGGATCCGTCGGCGTAAAGTCTGAGAAA