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Temporal expression patterns of tcpP, toxT, ctxA, and tcpA, as determined by primer extension. Cultures were grown as described in the legend to Fig. 3 by using a helium atmosphere. Expression of genes was determined hourly. (A) Expression pattern of tcpP; (B) expression pattern of toxT; (C) expression pattern of ctxA; (D) expression pattern of tcpA. In each case a DNA sequence ladder (GATC) was included to identify the putative transcriptional start site.
