Skip to main content
. Author manuscript; available in PMC: 2012 Sep 17.
Published in final edited form as: Transfusion. 2008 Sep 16;48(12):2638–2644. doi: 10.1111/j.1537-2995.2008.01926.x

Fig. 1.

Fig. 1

LTC-IC colony on UC-MSCs. UC-MSCs were observed to have the capacity to support the maintenance of hematopoietic colonies. (A) Shown is a typical monolayer of UC-MSCs (Passage 5) that was subsequently utilized for LTC-IC assays and (B) a typical LTC-IC colony formed when human cord blood CD34+ cells are grown on the UC-MSCs for 35 days. These colonies can then be detached using trypsin-ethylenediaminetetraacetate, placed in single-cell suspensions, and replated for myeloid progenitor colony formation in methylcellulose. Myeloid colonies (C) then form after an additional 14 days of methylcellulose culture. Magnification, 400×.