LTC-IC assay. The numbers of CFCs per 1 × 105 CD34+ cord blood cells were assessed after 35 days of culture on either BM-MSCs or UC-MSCs followed by 14 days of myeloid progenitor colony formation in methylcellulose medium. Different MSC donors are represented by numbers (1–3) and different segments of the cord from the same donor are represented by letters (A–C). Some UC-MSC samples were observed to be capable of supporting cord blood LTC-IC formation at levels comparable to BM-MSCs (UC-MSC 1A, 1C, 3A) while other UC-MSCs were not (UC-MSC 1B, 2B, 2C, 3B). The ability to support LTC-IC cultures for all donor samples was statistically compared to BM-MSCs (1A) utilizing a t test (*p ≤ 0.05, n = 3 for each sample).