FIG. 3.

Northern blot analysis of the abnA- and xsa-specific transcripts. Ten micrograms of total RNA extracted from the wild-type strain grown in the absence of sugar (−), in the presence of arabinose (Ara), or in the presence of arabinan (Arab) was run in a 1.2% (wt/vol) agarose formaldehyde denaturing gel (see Materials and Methods). The RNA ladder used as molecular size markers is indicated to the right of each gel. The abnA-specific (left) and xsa-specific (right) transcripts detected with DNA probe fragments abnA (767 bp) and xsa (1,420 bp) are indicated by heavy arrows. The additional weak high-molecular-weight RNA signal visible with the abnA DNA probe (left) is indicated by a light arrow.