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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: Cell. 2012 Aug 31;150(5):895–908. doi: 10.1016/j.cell.2012.08.002

Figure 4. Characterization of inhibition by modified ss-siRNAs.

Figure 4

Western analysis of inhibition of HTT expression by:

(A) ss-siRNA 553822 (mismatched base at P9) containing a 5'-phosphate;

(B) ss-siRNA 557426 containing three central mismatches;

(C) ss-siRNA 556888 containing a mismatch at P6; and

(D) ss-siRNA 553822 (mismatched base at P9) in 44-CAG-repeat GM04719 cells.

(E) Effect of ss-siRNA 537775 on other genes containing trinucleotide repeats.

MM: an RNA containing multiple mismatches. Western analysis for parts A–C is representative data from three or more experiments and error bars are standard error of the mean (SEM).