Figure 6. Allele-selective inhibition of HTT by ssiRNA in Q150 HD mouse model.
(A) Western analysis of HTT expression on allele-selective inhibition by ss-siRNA 537775 (n=5) in Q150/Q7 mouse frontal cortex.
(B) Quantitation of wild-type and mutant HTT protein levels shown in (A).
(C) Q-PCR analysis of HTT mRNA levels in mouse frontal cortex after treatment with ss-siRNA, vehicle, or control gapmer ASO.
(D) Western analysis HTT expression after allele-selective inhibition by ss-siRNA 537773 (n=5) in different brain regions.
(E) Quantitation of western analysis from (D). Results from each treatment group/brain section were averaged.
Error bars represent standard error of the mean (SEM) after averaging quantitation results from multiple gel images. *p < 0.05; **p < 0.01; ***p < 0.001.
See also Figure S3.