Figure 3. Formation of Distinct LCMV-Specific Effector CD4⁺ T Cells Subsets.

(A and B) Stg chimeric mice were infected with LCMV and 8 (A) or ~30 (B) days p.i. splenocytes were analyzed by flow cytometry for expression of the indicated proteins. PSGL1^hiLy6C^hi (red), PSGL1^hiLy6C^lo (blue), and PSGL1^loLy6C^lo (green) Stg CD4⁺ T cells were gated and the average MFI + SD for the various proteins are shown in bar graphs. CD4⁺ T cells were also stimulated with GP_61–80 peptide in vitro and the average frequency of IFN-γ⁺ and IL-2⁺ Stg T cells + SD are shown.

Data shown are representative of three independent experiments (n = 3 per group).

(C) Stacked bar graph of Stg CD4 T cells (left) or CD44^hi polyclonal CD4⁺ T cells (right) – SD in each subset after LCMV infection.

(D) Total number of Stg CD4⁺ T cells + SD of each subset during LCMV infection. Cumulative data from 9–37 mice/time point from more than 20 independent experiments are graphed.

(E) The expression of CD62L and CCR7 on Ly6C^hi and Ly6C^lo Stg CD4⁺ T cells at day 60 p.i. is shown in FACS plots. See also Figure S2.