TABLE 2.
Nystatin production by recombinant S. noursei strains with inactivated or overexpressed regulatory genes
| Strain or background (genotype) | Complementation | Nystatin production (% of WT)a |
|---|---|---|
| SR12 (ΔnysRI) | 0.5 | |
| pNRE2 (ermE*p::nysRI) | 59 | |
| SR34 (ΔnysRII) | 7 | |
| pC3A1 (ermE*p::nysRII) | 100 | |
| SR56 (ΔnysRIII) | 9 | |
| pNRT3 (ermE*p::nysRIII) | 100 | |
| NR4K (nysRIV::Kmr) | 2 | |
| pNR4ES (ermE*p::nysRIV-S) | 2.5 | |
| pNR4EL (ermE*p::nysRIV-L) | 57 | |
| ATCC 11455 (WT) | pSOK804 | 100 |
| pNRE2 (ermE*p::nysRI) | 104 | |
| pC3A1 (ermE*p::nysRII) | 121 | |
| pNRT3 (ermE*p::nysRIII) | 100 | |
| pNR4EL (ermE*p::nysRIV) | 136 |
a
Values are means from three independent experiments. In general, variations were within 7% of the mean.