TABLE 2.
Primers used for RT-PCR and for quantitiative real-time PCR to analyze transcriptional organization and expression of genes dppA-E, oppA1-F, and dpsA in S. agalactiae
| Transcriptional region or gene tested | Gene(s) hybridized | First primer (5′→3′) | Second primer (5′→3′) |
|---|---|---|---|
| dpp region | gbs0183-dppA | ATGACTATGGACACCTTG | GCAGGATTAGGTCTAGGG |
| dppA-dppC | CCTTGAGCACCTTTCCCAAC | TAGCGGAGCAAGCTAAAGC | |
| dppB-dppE | CTCGACTTGGGTAATAAG | GTGATACACCGCTACTTC | |
| dppD-gbs0189 | GGTATCATTCTGTCAGAGGC | AAATCTCATGCGTGTGGCAC | |
| opp region | gbs0143-oppA1 | CAGTATAGCGCCTTTTTTC | GCAACTGAAGCAAGTGTAAGG |
| oppA1-oppB | ATCATACGTGATACTGGCTG | ACTCTACTTCCGTAGTGG | |
| oppB-oppC | ACGTTGACGTTCACTCTCC | GCTATCTTGATAACAGACG | |
| oppC-oppF | GATGGACTTCTATGTCTCG | ACGTTGACGTTCACTCTCC | |
| oppF-rDNA | AGCTATTGAGCAAGACGGAC | TCAGACTTCCGTCCATTGC | |
| dpsA region | gbs1512-dpsA | TGGACATTAGACAACTACG | CGAAAATAGCACGCATACC |
| dpsA-dpsA | GGTATGCGTGCTATTTTGC | CTGATAATCCAACTGGTGAG | |
| dpsA-gbs1514 | CTCACCAGTTGGATTATCAG | CATTGGTACACAGATGG | |
| dppA gene | dppA | TAGCGGAGCAGCTAAAGC | GCAGATTCATCCCAAGTC |
| dppB gene | dppB | GATCGAATTGTAAGGTGG | AGAAGTAGCGGTGTATCAC |
| dppE gene | dppE | CTCCTAGATCTGGTGAAG | CCATTGAAAGATACGATACG |
| oppA1 gene | oppA1 | TATGTTGCCCCAGGTTATG | GTTATAAGCGCCTTGCTC |
| oppB gene | oppB | CGCTAGGGATTTCTATGC | CGTCTGTTATCAAGATAGC |
| oppF gene | oppF | CGTGATATCGTAGCAGAAGG | ACGTTGACGTTCACTCTCC |
| dpsA gene | dpsA | GGTATGCGTGCTATTTTGC | CCAATAGCAGCAAGTGAG |