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. 2012 Jul 25;96(1):109–119. doi: 10.1093/cvr/cvs238

Figure 4.

Figure 4

Co-expression of filamin-A, TG2, SERT, and Tph1 during foetal cardiac valve maturation. IHC's were performed to determine overlap of the expression of filamin-A, TG2, SERT, and tph1 during cardiac development. For each immunostain, MF20 is green, nuclei-blue, and specific protein is in red. (A) At E13.5, no significant overlap of expression is observed between each of the proteins. No detectable expression of TG2 is seen. Only punctate expression of SERT is observed in the developing valves (arrows), as well as expression in the endocardium and myocytes (arrow heads). Tph1 is expressed in the developing cushions but is restricted to the endocardium and subendocardial mesenchyme (arrows) filamin-A is expressed in all non-myocyte cells at this stage. (B) By E17.5, each protein shows significant overlap in expression patterns being primarily restricted to the interstitial valve fibroblast. Scale bars: low magnification = 200 µm, high magnification = 100 µm.