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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: J Am Chem Soc. 2012 Aug 27;134(36):14789–14799. doi: 10.1021/ja3034137

Figure 1.

Figure 1

Alignment of the amino acid sequences for the EGFP, mOrange, mOrange2, PSmOrange and PSmOrange2 proteins. Alignment numbering follows that of EGFP. Residues buried in the protein β-barrel fold are shaded. Asterisks indicate residues that form the chromophore. Mutations in PSmOrange relative to PSmOrange are indicated in white on black background.