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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: J Am Chem Soc. 2012 Aug 27;134(36):14789–14799. doi: 10.1021/ja3034137

Figure 3.

Figure 3

PSmOrange2 in live HeLa cells. (A) Cells expressing PSmOrange2 fusions with indicated proteins before (Pre) and after (Post) photoswitching. Images in orange (green) and farred (red) channels are shown. Scale bar, 10 μm. (B) Flow cytometry analysis of cell populations expressing PSmOrange2 (blue and cyan lines) and PSmOrange (red and green lines) before (Pre) and after (Post) photoswitching. The 638 nm excitation laser and 660/20 nm emission filter were used. (C) Mean far-red fluorescence intensities of PSmOrange2 and PSmOrange expressing cells at indicated photoswitching times. The 647 nm excitation laser and 670/30 nm emission filter were used. Error bars, SD.