FIG. 3.

GH18_N and GH18_C have similar pH and temperature optima. To determine the pH optimum (top panel) and temperature optimum (bottom panel) for each catalytic domain, GH18_N (triangles) and GH18_C (squares) were purified as described in Materials and Methods and incubated with MUF-diNAG or MUF-triNAG, respectively, as described in footnote a of Table 1. The activity under reaction conditions that permitted maximum activity was assigned a value of 100%. The data are the means from three replicates, and each experiment was repeated three times with similar results. Standard errors are indicated by error bars.