TABLE 1.
Activities of polypeptides containing ChiB catalytic domains on different MUF analogs
| Domain | Rate of MUF release (nmol/min/mg of enzyme)a on:
|
Ratio of ratesb | |
|---|---|---|---|
| MUF-diNAG | MUF-triNAG | ||
| GH18N | 80 ± 1.04 | 5.9 ± 1.77 | 13.6 |
| GH18C | 6.9 ± 0.311 | 19 ± 0.429 | 0.363 |
a
Polypeptides containing each active domain were purified as described in Materials and Methods, and 2 μg was incubated with the chitin analogs MUF-diNAG and MUF-triNAG. Reactions were stopped after 5 to 20 min, and the fluorescence of the reaction was measured. The rate of reaction was linear for at least 20 min. The data are the means and standard errors from three replicates, and each experiment was repeated three times with similar results.
b
Rate of MUF release from MUF-diNAG/rate of MUF release from MUF-triNAG.