TABLE 2.
Activities of polypeptides containing ChiB catalytic domains on native chitin
| Polypeptide components of reaction (pmol) | Rate of depolymerizationa | ||
|---|---|---|---|
| GH18N | GH18C | GH18N+C | |
| 250 | 0.0158 ± 0.0015 | ||
| 250 | 0.0340 ± 0.0017 | ||
| 250 | 250 | 0.1190 ± 0.0081b | |
| 250c | 0.0645 ± 0.0031 | ||
a
Rates are given as micromoles of reducing sugar released per minute per 250 or 500 pmol of catalytic domain (depending on the reaction components) Data are means and standard errors.
b
The theoretical calculated rate (0.0498 μmol/min/500 pmol of catalytic domain) was determined by adding the observed rate for each domain acting alone and assuming no cooperative interaction.
c
Each molecule of GH18N+C contains two catalytic domains; therefore, 500 pmol of catalytic domain was present.