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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Hepatology. 2011 Oct;54(4):1379–1387. doi: 10.1002/hep.24508

Figure 3. Uptake and processing of soluble ovalbumin protein and the level of MHC class I protein.

Figure 3

A. After 4 hours of incubation with fluorescein OVA conjugate (100 μg/ml) the cells were studies by flow cytometry. The data showed higher levels of OVA uptake in LSECs and lower levels in hepatocytes and HSCs. B. Processing of DQ™ OVA showed increased fluorescent signal in LSEC population and lower level of signal in hepatocytes. However this can be influenced by level of OVA uptake by these cells. C and D. After 24 hours of incubation we stained for both H-2Kb and H-2Kb-SIINFEKL proteins, respectively, and assessed by flow cytometry. The data showed the highest levels of H-2Kb on HSCs and lowest levels on hepatocytes. H-2Kb-SIINFEKL is highest in LSECs. HSCs also displayed high levels of H-2Kb-SIINFEKL. * P<0.05 from all other APCs. # P<0.05 from LSECs, KCs and DCs. § P<0.05 from hepatocytes, KCs and DCs. All data are from 3 independent experiments.