TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| E. coli strains | ||
| 1177 | UTI isolate (O1:K1:H7) | 7 |
| CN1016 | 1177 fim::kan | 7 |
| MG1655 | K-12 reference strain | 2 |
| MS427 | MG1655 flu | 33 |
| MS428 | MG1655 fim | 22 |
| MS528 | MG1655 flu fim | 22 |
| MS641 | MG1655 oxyR::kan | This study |
| DD14 | MS427 containing pKKJ128 and pBR322 | This study |
| DD19 | MS641 containing pBR322 | This study |
| DD26 | MS641 containing pKT274 | This study |
| DD27 | MS641 containing pIR100 | This study |
| DD34 | MS427 containing pACYC184 and pKT274 | This study |
| DD35 | MS427 containing pACYC184 and pIR100 | This study |
| DD36 | MS427 containing pACYC184 and pBR322 | This study |
| DD37 | MS427 containing pKKJ128 and pKT274 | This study |
| DD38 | MS427 containing pKKJ128 and pIR100 | This study |
| MS718 | MS428 containing pFCH2235 | This study |
| PK1081 | CN1016 containing pFCH2235 | This study |
| K. pneumoniae strains | ||
| C105 | K35 capsule | 45 |
| C105 NCV | Spontaneous C105 NCV | 45 |
| LH71 | C105 containing pIB264 | This study |
| LH72 | C105 NCV containing pIB264 | This study |
| PK1013 | C105 containing pHHA147 | This study |
| PK1014 | C105 NCV containing pHHA147 | This study |
| PK1016 | C105 containing pBR322 | This study |
| PK1021 | C105 NCV containing pBR322 | This study |
| Plasmids | ||
| pFHC2235 | IPTG-inducible dam gene in pUC19a | Gift from F. G. Hansen |
| pHHA147 | flu gene from MG1655 in pBR322 | 38 |
| pIR100 | E. coli K5 capsule gene operon in pBR322(cos4) | Gift from I. Roberts |
| pKKJ128 | flu gene from MG1655 in pACYC184 | 21 |
| pKT274 | E. coli K1 capsule gene operon in pHC79 | 13 |
| pKKJ101 | flu gene in pUCP22 | 21 |
| pIB264 | AIDA-I autotransporter and glycosylation genes in pBR322 | 4 |
a
IPTG, isopropyl-β-d-thiogalactopyranoside.