TABLE 1.
Identification of the protein spots that increase or decrease in intensity upon inactivation of the crc genea
| Spot(s) | Protein (gene) with best homologyb | Effect of Crcc |
|---|---|---|
| 1 | Cysteinyl-tRNA synthetase (cysS) | Stimulation |
| 2 | Malate-quinone oxidoreductase 2 (mqo-2) | Stimulation |
| 3 and 4 | Homogentisate 1,2 dioxygenase (hmgA) | Repression |
| 5 | Branched-chain amino acid ABC transporter (braC) | Repression |
| 6 and 9 | Sugar ABC transporter (PP1015) | Repression |
| 7 and 8 | Oxaloacetate decarboxylase alpha (oadA) | Repression |
| 10 | Probable CoA transferase, subunit A (PP3122) | Repression |
| 11 and 12 | 4-Hydroxyphenylpyruvate dioxygenase (hpd) | Repression |
| 13 | Probable CoA transferase, subunit B (PP3123) | Repression |
a
Spots indicated in Fig. 2 were excised from the gel and subjected to MALDI peptide mass fingerprinting.
b
Protein showing the best score. Score is −10·Log(P), where P is the probability that the observed match is a random event. Protein scores higher than 50 are significant. Only those higher than 80 were taken into account. All proteins belong to P. putida KT2440.
c
The intensity of spots 5 and 6 increased by about three- and eightfold, respectively, upon inactivation of crc. Spots 1 and 2 were undetectable in the strain lacking Crc, and spots 3, 4, and 7 to 13 were undetectable in the strain having a wild-type crc gene.