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Gel filtration analysis of MBP and MBP-rep. (Upper and middle panels) The mobility of MBP (upper panel) and MBP-rep (middle panel) during gel filtration yielded rS values for each protein. (Lower panel) Migration of the two proteins when they were subjected to gel filtration as a 1:1 mixture. The molecular masses (in kilodaltons) and elution times of the standard proteins are indicated at the top in each panel. mAU, milliabsorbance units.
