TABLE 1.
In vivo β-galactosidase-stimulating activities of CooA homologs
| CooA homolog | % β-Galactosidase activitya
|
Pellet colorb | |
|---|---|---|---|
| −CO | +CO | ||
| R. rubrum | 1.1 | 100 | Red |
| A. vinelandii | 11 | 500 | Light brown |
| A. vinelandii (S77C) | 56 | 493 | Light brown |
| C. hydrogenoformans 2340 | 2.0 | 1,268 | Red |
| C. hydrogenoformans 2340 (C80S) | 1.4 | 903 | Red |
| C. hydrogenoformans 2340 (S)c | 1.0 | 1,065 | Red |
| D. vulgaris | 1.5 | 3.8 | Pale brown |
| D. desulfuricans | 1.3 | 30 | Negatived |
| D. hafniense | 1.0 | 338 | Pale brown |
| C. hydrogenoformans 2350 | 1.0 | 1.5 | Negative |
a
Activity indicates the mean value of the results from two independent assays expressed relative to that of R. rubrum CooA in the presence of CO. The assays showed a variability of <10%.
b
Cell pellet color obtained from anaerobically grown cultures.
c
Shorter version of cloned C. hydrogenoformans 2340 CooA.
d
Negative indicates a similar pellet color (gray) to that of the control strain which contains only vector.