TABLE 2.
Heme accumulation and Soret maxima of selected CooA homologs
| CooA homolog | Concn (nM) of heme b per 1 mg of protein/mla
|
Soret maxima (nm) for form
|
|||
|---|---|---|---|---|---|
| Aerobic growth | Anaerobic growth | Fe(III) | Fe(II) | Fe(II)-CO | |
| pEXT20b | <50 | <50 | |||
| R. rubrum CooA | 4,292 | 1,362 | 424 | 425 | 422 |
| A. vinelandii CooA | 145 | 357 | 419 | 421.5 | 419.5 |
| C. hydrogenoformans 2340 CooA | 773 | 1,656 | 416 | 424.5 | 420.5 |
a
The heme b content was quantified by a modified pyridine-hemochromogen method with partially purified samples as described in Materials and Methods.
b
pEXT20 represents the same preparation of a control strain containing only the cloning vector.