TABLE 2.

DNA probes used in this study

Probe	Descriptiona
mdh-P	817-bp SpeI fragment of pBM19 covering 774 bp of the 5′-terminal region of mdh and upstream sequences
pfk-P1	766-bp EcoRV-BglII fragment of pBM19 covering 625 bp of the 5′-terminal region of pfk and upstream sequences
tkt-P	1,008-bp PstI fragment of pBM19 covering the central coding region of tkt
fba-P	561-bp AvaI fragment of pBM19 covering the central coding region of fba
rpe-P	526-bp ApoI fragment of pBM19 covering 494 bp of the 5′-terminal region of rpe and upstream sequences
glpX-P	483-bp HindIII fragment of pBM19 covering the central region of glpX
pfk-P2	800-bp PCR fragment obtained by using primers pfk-F and pfk-R covering the central region of the pBM19 pfk gene
repB-P	989-bp PCR fragment obtained by using primers repB-F and repB-R covering the central region of the pBM19 repB gene
tkt/fba-P	780-bp PCR fragment obtained by using primers fba/tkt-F and fba/tkt-R covering the 3′ end of tkt and the 5′ end of fba
rmp-P	Corresponding to the 925-bp RMP insert of plasmid pRMP3

^aThe DNA probes were labeled with DIG as described in Materials and Methods. The AT contents of the resulting DNA probes were between 58.9 and 63.9%.