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Effects of various inhibitors on proteolytic activity of purified enzymea
| Inhibitor (concn) | Relative activity (%) |
|---|---|
| None (control) | 100 |
| E-64 (10 μM) | 94 |
| Leupeptin (100 μM) | 107 |
| Iodoacetic acid (1 mM) | 80 |
| TLCK (100 μM) | 27 |
| TPCK (100 μM) | 17 |
| PMSF (1 mM) | 0.5 |
| DFP (100 μM) | 8 |
| EDTA (10 mM) | 90 |
| Pepstatin A (1 μM) | 94 |
The inhibitors were tested at their maximum effective concentrations. Activity against azoalbumin in the absence of inhibitors was taken as 100% (control).
