Table 1.
Incubation method | 111In3+labelling in % | Eu3+labelling in % |
---|---|---|
2.4-fold excess of linker 4 |
46.2 |
36.7 |
8-fold excess of linker 4 |
53.1 |
36.2 |
24-fold excess of linker 4 |
58.6 |
41.7 |
for comparison: |
|
|
cys-anxA5 5 | 0.0 | 0.0 |
In case of europium the yield was determined by measuring the fluorescence, in case of 111In3+ by radioactivity measurement. The numbers are percentages of labelled protein.