Figure 3 .
IRF5 promotes thyroid cancer cells proliferation. A. The specified cell lines were transduced with either EV or IRF5-GFP and analyzed for infection efficiency with an anti-GFP immunoblot. B. 5 × 103 thyroid cancer cells described in A were plated in the absence of drugs for 24 (white columns), 48 (light grey columns), 72 (dark grey columns) and 96 hours (black columns) and analyzed for their proliferation. Dashed lines indicate baseline proliferation rates for each cell line assessed 4 hours after plating and arbitrarily set at 100%. C. The same thyroid cancer cells were implanted in 24-well plates and, after 72 hours, employed to calculate their doubling time using the doubling time online calculator (http://www.doubling-time.com/compute.php). p-values with 95% confidence intervals were obtained using t-tests determined with the Prism Software (GraphPad Software, *p < 0.05, **p < 0.01, *** p < 0.001).