TABLE 2.
Frequency of amino acids in HIV-2 protease, from untreated patients, at positions associated with PI resistance in HIV-1
| Virus | HIV-2 protease amino acid at positiona:
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10 | 20 | 24 | 30 | 32 | 33 | 36 | 46 | 47 | 48 | 50 | 53 | 54 | 63 | 71 | 73 | 77 | 82 | 84 | 88 | 90 | |
| Wild-type HIV-1b | L | K | L | D | V | L | M | M | I | G | I | F | I | L | A | G | V | V | I | N | L |
| Drug-resistant HIV-1c | F, I, R, V | M, R | I | N | I | F | I | I, L | V | V | V, L | L | L, M, V | P | V, T | S, A | I | A, F, T, S | V | S, D | M |
| HIV-2 subtype A (con- sensus sequence)d | V | V | L | D | I | V | I (V) | I (V) | V | G | I | F | I | E | V | A | T | I | I | N | L |
| HIV-2 subtype B (con- sensus sequence)d | V | V | L | D | I | V | I | I | V | G | I | F | I | E | V | A | T | I | I | N | L |
| HIV-2 strains from untreated patientc | V (100) | V (94), I (6) | L (100) | D (100) | I (100) | V (100) | I (82), V (12), V/I (6) | I (59), V (35), T (6) | V (100) | G (100) | I (100) | F (100) | I (100) | E (100) | V (94), I (6) | A (100) | T (100) | I (100) | I (100) | N (100) | L (100) |
Below each amino acid position is the list of amino acids that are observed and in parentheses is the percentage of sequences concerned. The predominant amino acid for each position is given first. Amino acids that are the most frequently found or always present in HIV-2 strains and are associated with drug resistance in HIV-1 are in bold type.
Wild-type sequence from the reference HIV-1 strain HXB2 (GenBank accession number K03455).
According to the International AIDS Society (IAS; http://www.iasusa.org/resistance_mutations/resistance.pdf).
Available at http://hiv-web.lanl.gov/content/hiv-db/ALIGN_CURRENT/ALIGN-INDEX.html; nonpredominant amino acids are indicated in parentheses.