Table 2.

Functional properties of recombinant nAChRs expressed in Xenopus oocytes

Subunits	EC 50(μM or nM)*	Hill slope	n  **	I max[ I mean] † (nA)
Dα5	8.8 ± 2.5 μM	1.1 ± 0.3	6	200 [141 ± 25]
Dα6	–	–	‡	–
Dα7	6.7 ± 1.7 μM	1.0 ± 0.3	4	86 [45 ± 13]
Dα5 + Dα6	8.6 ± 2.4 μM	1.0 ± 0.1	5	47 [20 ± 8]
Dα5 + Dα7	1.6 ± 0.3 μM	1.0 ± 0.1	3	53 [39 ± 8]
Dα6 + Dα7	–	–	‡	–
Dα5 + Dα6 + Dα7	13.5 ± 1.7 nM*	1.2 ± 0.3	6	150 [107 ± 12]

Note, in all cases, nAChR subunits were co-expressed with CeRIC-3.

* Note, EC₅₀ value for Dα5 + Dα6 + Dα7 is expressed as nM, rather than μM.

**EC₅₀ and Hill slopes are means ± SEM of separate fits to dose–response curves derived from independent experiments (n = 3–6).

† Relatively small currents were detected with all subunit combinations, as indicated by the size of the maximum current that was detected with each subunit combination (I_max) and the mean maximum current (I_mean) from between 6–20 different oocytes.

‡ Subunit combinations that failed to generate functional responses are indicated by a dash. This is based on studies conducted with at least 5 batches of oocytes that generated functional nAChRs with other subunit combinations and at least 10 oocytes from each batch (n > 50).