Figure 3. Lifespan-shortening effects of ubiquitous adult-induced expression of UAS-GFPRNAi are diet-dependent.

Mated female tubGS>GFPRNAi flies were reared on 1 SYA food and placed on different foods containing increasing amounts of yeast (0.1× to 2× relative to standard food) either containing RU486 (shades of red) or not (shades of grey) from day two of adulthood. A The resulting lifespans. B The median survival times, with asterisks indicating significant differences in survival between the induced (+ RU486) and uninduced (-RU486) conditions (Log-rank test, p<0.001, n =  ∼150 per condition). The survival between induced and uninduced at 0.5× yeast was not significant (Log-rank test, p>0.5, n =  ∼150 per condition). Cox Proportional Hazards analysis revealed significant effect of yeast quantity, presence of RU486 and their interaction (p<0.0001).