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. 2012 Sep 17;7(9):e45224. doi: 10.1371/journal.pone.0045224

Figure 5. The analysis of uterine decidualization-associated molecules.

Figure 5

A and B: the relative expression of target proteins in the D7 uteri of mice immunized with pCR3.1-IGFBP7-t, the pCR3.1 vector or saline. The black columns represent the relative expression value in mice immunized with pCR3.1-IGFBP7-t, whereas the grey columns represent the mice immunized with pCR3.1, and the white columns indicate the mice immunized with saline. After immunization with pCR3.1-IGFBP7-t, the expression of Bcl-2, caspase3, IGFBP1 and VEGF were significantly decreased. No significant variation of Bax, FAS or FAS-L was observed. C: Reduced expression of VEGF in mice uteri at D7 after immunization with pCR3.1-IGFBP7-t. Brown staining represents the positive signals for VEGF, which was found to be reduced after DNA immunization. De: Decidua; Em Embryo. Bar represents 250 µm in a1, b1 and c1 and 50 µm in a2, b2 and c2. D: The quantitation of IGFBP1 in uterus by ELISA. After immunization with pCR3.1-IGFBP7-t, the expression of IGFBP1 in uterus was decreased to about 5.54 ng/1 µg uterine protein. *: P<0.05.