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. 2012 Sep 17;7(9):e45324. doi: 10.1371/journal.pone.0045324

Figure 8. The individual adaptin subunit failed to localize at Golgi/endosomes in sip1-i4 mutant cells.

Figure 8

Subcellular localization of Apm1-GFP (A), Aps1-GFP (C), GFP-Apl2 (B), and GFP-Apl4 (D) in wild-type (wt) and sip1-i4 mutant cells (sip1-i4). Cells expressing various GFP-tagged adaptin subunits were cultured in YPD medium at 27°C were incubated with the dye FM4-64 for 5 min at 27°C to visualize the Golgi/endosomes. The fluorescence of the FM4-64 was examined under the fluorescence microscope. Arrowheads indicated the localization of GFP-tagged adaptin subunits to the Golgi/endosomes. Bar, 10 µm.