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. 2012 Sep 17;7(9):e45102. doi: 10.1371/journal.pone.0045102

Figure 1. Generation and analyses of CD11c- and control-scFv-IDRgag fusion proteins.

Figure 1

(A) Schematic representation of CD11c and control-scFv constructs fused to IDRs of either FV or OVA containing immunodominant epitopes. Abbreviations: vds (vector derived sequence); iegr (factor Xa cleavage site); IDR (immunodominant region). (B) The recombinant CD11c- and control-scFv-IDRgag (lanes 4, 5, 6 and 1, 2, 3, respectively) were expressed in the bacteria as inclusion bodies, which were isolated and dissolved in 8 M urea. The proteins were purified using a HisTrap HP column. Purified recombinant proteins were then refolded by slow dialyses. The verification of the purified proteins was performed using SDS-PAGE (left) followed by Western blotting (right). (Lanes 1 and 4 bacterial lysate; lanes 2 and 5 isolated inclusion bodies dissolved in 8M urea; lanes 3 and 6 purified, refolded proteins; M molecular weight marker).